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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -LAP2 (Lamina Associated Polypeptide 2, Thymopoietin, TMPO)

Anti -LAP2 (Lamina Associated Polypeptide 2, Thymopoietin, TMPO)

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Specifications

Clone Host Grade Applications
Monoclonal Hamster Purified B IP IF
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
Catalog #L1241
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Cellular Localization  
Nuclear and Secreted
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4i386
HostHamster
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityIgG fraction.
ImmunogenPore complex-lamina fraction isolated from rat liver nuclear envelopes.
SpecificityDetects Lamin Associated Polypeptide (LAP) 2 from various rat tissues but does not crossreact with LAP 1A, 1B & 1C. Recognizes a single 53kD protein representing LAP 2 in rat liver nuclear envelope fractions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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