| Versican is a large multi-domain chondroitin sulfate proteoglycan. It is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. Versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent Mr of ~1000kD, of which the core contributes 400kD from the 2409 amino acids. The 12-15 covalently attached chondroitin sulfate chains make up the rest. |
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| Catalog # | L1350-01 |
| Applications | Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (Formalin fixed, paraffin embedded): 10ug/ml. Tissues must be treated with Chondroitinase (PBS + 0.5U/ml of Chondroitinase) for 30 min. at RT prior to blocking and staining. |
| Western Blot: 0.5ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Positive control (IHC) | Surrounding cartilage, neural tube brain. |
| Positive control (WB): Newborn mouse brain |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Companion Product | C5068: Chondroitinase ABC |
| C5068-05: Chondroitinase ABC Protease Free |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS. |
| Purity | Purified by affinity chromatography. |
| Immunogen | GST fusion protein containing aa535-598 of mouse versican. |
| Specificity | Recognizes mouse versican GAG alpha domain. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
