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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Large Proteoglycan, GAG alpha Domain (Versican, Versican Core Protein, Large Fibroblast Proteoglycan, Chondroitin Sulfate Proteoglycan Core Protein 2, Chondroitin Sulfate Proteoglycan 2, PG-M, Vcan, Cspg2)

Anti -Large Proteoglycan, GAG alpha Domain (Versican, Versican Core Protein, Large
Fibroblast Proteoglycan, Chondroitin Sulfate Proteoglycan Core Protein 2, Chondroitin
Sulfate Proteoglycan 2, PG-M, Vcan, Cspg2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, Versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ~1000kD, of which the core contributes 400kD from the 2409 amino acids, and the 12-15 covalently attached chondroitin sulfate chains make up the rest.
Catalog #L1350-01
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunohistochemistry (FFPE): 10ug/ml. Tissues must be treated with Chondroitinase before staining (0.5U/ml in 100mM Tris-HCL, pH 7.3 for 30 minutes at RT) prior to blocking and staining.
Western Blot: 0.5ug/ml.
Optimal dilutions to be determined by the researcher.
Positive Control (IHC) Surrounding cartilage, neural tube brain.
Positive Control (WB)Newborn mouse brain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS.
PurityPurified by affinity chromatography
ImmunogenGST fusion protein containing aa535-598 of mouse versican.
SpecificityRecognizes mouse versican GAG alpha domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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