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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Large Proteoglycan (Versican)

Anti -Large Proteoglycan (Versican)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Versican (versatile proteoglycan; also known as PG-M) is a 500-600kD, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa21-346), two large, alternately spliced GAG attachment domains (GAGalpha; aa348-1335; GAGbeta aa1336-3088) and a C-terminal selectin-like region (G3; aa3089-3396). Over aa1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Catalog #L1350-03
ApplicationsSuitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml detects Versican in recombinant human Versican isoform V0.
Immunocytochemistry: 5-15ug/ml detects Versican in immersion fixed HeLa cells.
Immunohistochemistry: 5-15ug/ml detects Versican in immersion fixed frozen sections of mouse embroyo.
Optimal dilutions should be determined by each laboratory.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611). Species Sequence Homology: canine (80%), mouse (60%).
SpecificityRecognizes human Large Proteoglycan. Species Crossreactivity: Mouse (IHC).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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