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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH IC

Versican (versatile proteoglycan; also known as PG-M) is a 500-600kD, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa21-346), two large, alternately spliced GAG attachment domains (GAGalpha; aa348-1335; GAGbeta aa1336-3088) and a C-terminal selectin-like region (G3; aa3089-3396). Over aa1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Catalog #	L1350-03
Applications	Suitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 0.1ug/ml detects Versican in recombinant human Versican isoform V0.
	Immunocytochemistry: 5-15ug/ml detects Versican in immersion fixed HeLa cells.
	Immunohistochemistry: 5-15ug/ml detects Versican in immersion fixed frozen sections of mouse embroyo.
	Optimal dilutions should be determined by each laboratory.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.2mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611).
Specificity	Recognizes human Versican. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.