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You are here:Home » Antibodies » Abs to Cell Markers » Anti -LAT, phosphorylated (Tyr132) (Linker for activation of T Cells)

Anti -LAT, phosphorylated (Tyr132) (Linker for activation of T Cells)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Linker for activation of T cells (LAT) is a 36kD transmembrane adapter protein that is tyrosine-phosphorylated following T-cell receptor (TCR) stimulation by ZAP-70 and Syk. Four distal tyrosine residues (Y132, Y171, Y191 and Y226 in human LAT) are crucial for its activity and subsequent signaling to downstream molecules. Tyrosine 132 mediates LAT binding to PLC-y-1 and is necessary for TCR-mediated calcium mobilization and activation of ERK and NFAT proteins.
Catalog #L1390-01C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat E6.1 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after recipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human LAT that contains tyrosine132. Species sequence homology: mouse (85%) and rat (77%).
SpecificityRecognizes human LAT when phosphorylated at Tyr132.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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