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You are here:Home » Antibodies » Abs to T Cells » Anti -LAT (Linker for activation of T Cells)

Anti -LAT (Linker for activation of T Cells)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).
Catalog #L1390-01D
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:40
Western Blot: 1:500
Immunohistochemistry: 1:100
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k246
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT
SpecificityRecognizes human LAT
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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