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You are here:Home » Antibodies » Abs to Cell Markers » Anti -LAT, phosphorylated (Tyr191) (Linker for activation of T Cells)

Anti -LAT, phosphorylated (Tyr191) (Linker for activation of T Cells)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Linker for activation of T cells (LAT) is a 36kD transmembrane adapter protein that is tyrosinephosphorylated following T-cell receptor (TCR) stimulation by ZAP-70 and Syk. Four distal tyrosine residues (Y132, Y171, Y191 and Y226 in human LAT) are crucial for its activity and subsequent signaling to downstream molecules. Tyrosine 191 has been shown to play a key role in T cell activation and to mediate Grb2 binding and Gads recruitment, thus mediating SLP-76 binding.
Catalog #L1390-02A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlStimulated Jurkat E6.1
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human LAT that contains tyrosine 191.
SpecificityRecognizes human LAT when phosphorylated at Tyr191. Species sequence homology: mouse and rat (92%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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