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You are here:Home » Antibodies » Abs to T Cells » Anti -LAT (36kD Phospho-tyrosine Adapter Protein, LAT1, Linker for Activation of T-cells Family Member 1, p36-38, pp36)

Anti -LAT (36kD Phospho-tyrosine Adapter Protein, LAT1, Linker for Activation of T-cells
Family Member 1, p36-38, pp36)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
LAT (Linker for activation of T cells; also pp36) is a 36-38kD type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TcR, GPVI, and FceRI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262aa in length. It contains a four aa extracellular segment with a 235aa cytoplasmic domain (aa28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36aa upstream of the standard site that may be accompanied by a deletion of aa114-142. There is also a deletion of aa88-97, and three substitutions: two aa for aa215-262, 38aa for aa55-262, and 50aa for aa35-262. Over aa30-113, human LAT shares 73% aa identity with mouse LAT.
Catalog #L1390-04D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived recombinant human LAT (aa30-113; Accession #O43561).
SpecificityRecognizes human LAT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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