Anti -LAT, CT (Linker for Activation of T Cells, Linker for Activation of T cells Family
Member 1, LAT1, 36kD Phospho-tyrosine Adapter Protein, p36-38, pp36)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
IH |
|
| The adaptor molecule LAT (linker for activation of T cells) is a palmitoylated integral membrane protein of 36-38kD, localized to the glycolipid-enriched microdomains in the plasma membrane and plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. Upon TCR engagement, LAT becomes phosphorylated on multiple tyrosine residues by ZAP-70 and Syk PTKs and binds Grb2, PLC-gamma1, the p85 subunit of PI3K, and other critical signaling molecules, thereby recruiting these molecules to the plasma membrane. It is required for TCR-mediated Ca2+ mobilization and optimal tyrosine phosphorylation of phospholipase C-gamma1, Vav and SLP-76. It is also required for Erk activation, CD69 up-regulation, and AP- and NFAT-mediated gene transcription. The binding of these proteins to LAT appears to be critical to TCR-mediated signaling. It is usually expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. LAT usually exists in two known isoforms. | | | Catalog # | L1390-05E | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (paraffin): 1:25-1:50 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Tonsil | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11E258 | | Host | Mouse | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Supernatant | | Immunogen | Prokaryotic recombinant corresponding to region of 120aa with in the C-terminus of the LAT molecule. Cellular Localization: Membrane. | | Specificity | Recognizes human LAT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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