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Specifications

Rapid detection of E. coli from water and food samples by fluorogenic detection methods.
Catalog #	L1400-05
	Lauryl Sulfate Broth was formulated by Mallmann and Darby for detection of coliform bacteria in water. This is a selective medium because it contains sodium lauryl sulfate which inhibits Gram positive microorganisms. Lactose is added to detect rapid lactose fermentation by coliforms. The addition of 4- methylumbelliferyl-B-D-glucuronide (MUG) to Lauryl Sulfate Broth allows for the rapid detection (4-24 hours) of E. coli-When the incubated medium is observed for fluorescence using a long-wave UV light (365 nm) source. It can also detect anaerogenic E-coli strains. Most strains of E. coli produce B- glucuronidase which hydrolyzes MUG to the fluorogenic compound 4- methyl-umbelliferone. Studies by Feng and Hartman noted that most E coli strains (96%) and all enterotoxigenic E coli strains (100%) and a moderate number of Salmonella (17%) and Shigella (40%) stains produce B-glucuronidase. False positives can also be caused by Streptococcus sp.
Quality Control Summary	Expected cultural response after 18-24 hours at 35.0°C.
	Organism Result
	Escherichia coli ATCC 25922 Growth, Gas +, Fluorescence +
	Proteus mirabilis ATCC 12453 Growth, Gas -, Fluorescence -
	Staphylococcus aureus ATCC 25923 No Growth
Appearance	White to off-white crystalline powder
Solubility	Clear, light amber and complete
pH	6.8± 0.2