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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -LAX1 (Linker for Activation of X Cells)

Anti -LAX1 (Linker for Activation of X Cells)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
LAX1 (Linker for activation of X cells) is a 70kD member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44kD, it runs anomalously at 70kD in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59-398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1-29. Over aa 136-398, human LAX1 is 47% aa identical to mouse LAX1.
Catalog #L1440
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 10ul labels 1-5x10e5 cells in a total reaction volume 200ul. Tested using Jurkat cells. For intraellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS.
Direct ELISA: 0.5-1ug/ml The detection limit for rhLAX1 ~0.3ng/well.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography
ImmunogenRecombinant protein corresponding to aa136-398 from human LAX1, expressed in E. coli.
SpecificityRecognizes human LAX1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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