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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -LAX1 (Linker for Activation of X Cells)

Anti -LAX1 (Linker for Activation of X Cells)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
LAX1 (Linker for activation of X cells) is a 70kD member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44kD, it runs anomalously at 70kD in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59-398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1-29. Over aa 136-398, human LAX1 is 47% aa identical to mouse LAX1.
Catalog #L1440
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1ug/ml detection limit for rhLAX1 ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/mL detection limit for rhLAX1 ~ 5ng/lane and 0.5ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived, recombinant human LAX1. (aa 136 - 398)
SpecificityRecognizes human LAX1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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