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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Lck, p56, (Non-receptor Protein Tyrosine Kinase)

Anti -Lck, p56, (Non-receptor Protein Tyrosine Kinase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
Lck is a pivotal enzyme for normal T cell development and TCR-mediated signal transduction. Lck is physically associated with the cytoplasmic region of T cell specific molecules CD4 and CD8 and plays an important role in the phosphorylation of several proteins further downstream in the T cell signalling cascade.
Catalog #L1565-17
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry (frozen) and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: detects a band of approximately 56kDa in Jurkat cell lysates.Membrane permeabilisation is required
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher
HybridomaSpleen cells from immunized F344/DuCrj rats were fused with cells of the mouse P3U1 myeloma cell line.
Recommended Secondary ReagentsF(ab')2 Rabbit anti mouse IgG:FITC
F(ab')2 Rabbit anti mouse IgG:FITC
ImmunohistochemistryRecommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP
Recommended Negative ControlsMouse IgG1
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A428
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 22-36 of human Lck.
SpecificityRecognizes p56 lck (Lck), a 56kD cytosolic non-receptor protein tyrosine kinase that is primarily expressed in T lymphocytes and natural killer cells.
Species Cross ReactivityThis antibody shows no cross reactivity with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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