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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Lck Interacting Molecule (LIME, dJ583P15.4, FLJ20406, Lck Interacting Membrane Protein, Lck Interacting Transmembrane Adaptor 1, LIME1, LP8067, RP4 583P15.5)

Anti -Lck Interacting Molecule (LIME, dJ583P15.4, FLJ20406, Lck Interacting Membrane
Protein, Lck Interacting Transmembrane Adaptor 1, LIME1, LP8067, RP4 583P15.5)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
Catalog #L1566-05
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide ENVYESIKEMGL, representing the C-Terminus of the human protein (residues 258-269), according to NP_076173 (Mouse).
SpecificitySpecies Crossreactivity: Reacts with Mouse. Predicted to react with Rat due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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