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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Human Leptin is an 167aa residue, ~16kD, adipocyte derived, secreted protein. Human Leptin was cloned, expressed in E. coli and purified. The purity of Leptin is greater than 95% as determined by SDS-PAGE. The identity of protein has been confirmed by N-terminal sequence analyses. Endotoxin level in the final preparation is less than 1pg/ug of murine Leptin. Mouse leptin is about 85% homologous with human Leptin. Purified human Leptin has been shown to be biologically active in reducing body weight, food consumption, and plasma glucose levels in ob/ob and NZO mice.
Catalog #L1669-01B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000 (10-100ng of Leptin protein/well)
Western Blot: 1:1000-1:5000 (chemiluminescence)
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No11C130
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to human leptin.
SpecificityRecognizes human leptin. Species Crossreactivity: mouse and rat (weak).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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