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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #L1669-01D
ELISA: To detect hLeptin by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen af nity puri ed antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hLeptin.
Western Blot To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Reconstitution The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at-200C. When reconstituted in sterile water to a concentration of >0.2mg/ml the antibody is stable for at least six weeks at 2-40C.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.2.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hLeptin.
SpecificityRecognizes human Leptin.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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