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Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Catalog # | L1669-01E | | Applications | | | ELISA: To detect hLeptin by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hLeptin. | | Western Blot | To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3ng/lane, under either reducing or non-reducing conditions. | | Reconstitution | The biotinylated antibody should be reconstituted to a concentration of 50mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 40C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at-200C. Avoid more than one freeze-thaw cycle. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Form | Supplied as a lyophilized powder in PBS. | | Purity | Puri ed by af nity chromatography. | | Immunogen | Highly pure (>98%) recombinant hLeptin (human Leptin). | | Specificity | Recognizes human Leptin. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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