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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #L1669-01E
Applications  
ELISA: To detect hLeptin by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hLeptin.
Western Blot To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Reconstitution The biotinylated antibody should be reconstituted to a concentration of 50mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 40C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at-200C. Avoid more than one freeze-thaw cycle.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder in PBS.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hLeptin (human Leptin).
SpecificityRecognizes human Leptin.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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