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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #L1669-01E
ELISA: To detect hLeptin by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hLeptin.
Western Blot To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Reconstitution The biotinylated antibody should be reconstituted to a concentration of 50mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 40C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at-200C. Avoid more than one freeze-thaw cycle.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hLeptin (human Leptin).
SpecificityRecognizes human Leptin.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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