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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IP
Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.
Catalog #L1669-01G
ApplicationsSuitable for direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionDirect ELISA: 1:10,000 dilution will yield 0.8 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5C73
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2.
ReconstitutionReconstitute with 500ul sterile dH2O.
PurityPurified by ion exchange chromatography.
ImmunogenRecombinant human leptin. MW: 16kD
SpecificityRecognizes human Leptin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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