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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP RIA
Human Leptin (Anti-Obesity Protein) is the human homolog of murine Leptin, the protein product of the ob (obese) gene in mice which seems to be involved in appetite control. The murine ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. It targets the central nervous system, particularly hypothalamus, affecting food intake. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes.
Catalog #L1669-01V
ApplicationsSuitable for use in RIA, ELISA, Western Blot, Immunoprecipitation and Cell Staining. Other applications not tested.
Recommended DilutionImmunoprecipitation: 1:500-1:5000; Quantitatively immunoprecipitates Leptin at dilutions ranging from 1:500-1:5000 depending upon the amount of Leptin in a sample. Tested using double antibody precipitation and magnetic bead techniques.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, 10mg/ml BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitution with 100ul 40-50% glycerol, PBS, 10mg/ml BSA.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa1-24, of human Leptin covalently attached onto a carrier protein.
SpecificityRecognizes human Leptin [1-24]. Specific for the amino terminal of the human Leptin. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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