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You are here:Home » Antibodies » Abs to Leptin » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Leptin (anti-obesity protein) is the product of the ob (obese) gene in mice and is involved in appetite control. The gene is expressed in adipose tissue and it is thought to be important in the regulation of fat stores. Mice of ob/ob genotype develop a form of diabetes similar to type II (non-insulin dependent) diabetes.
Catalog #L1669-03
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:4000
Immunohistochemistry (frozen sections): 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS containing 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 25-44, QKVQDDTKTLIKTIVTRIND of mouse leptin.
SpecificityRecognizes mouse leptin. Species Crossreactivity: human, rat and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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