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Anti -Leptin (Ob, Obese Protein, Obesity Factor)
Anti -Leptin (Ob, Obese Protein, Obesity Factor)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B |
|
| Catalog # | L1669-06 | | Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilution | ELISA (Direct and Indirect): 1:5000-1:10,000 | | Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Incubate for 1 hour at RT. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. No preservative added. | | Purity | Purified. | | Immunogen | Recombinant mouse leptin. | | Specificity | Recognizes human (weak), mouse and rat (weak) leptin. Detects a band of ~16kDa by Western blot. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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