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You are here:Home » Antibodies » Abs to Leptin » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Catalog #L1669-06
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA (Direct and Indirect): 1:5000-1:10,000
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified.
ImmunogenRecombinant mouse leptin.
SpecificityRecognizes human (weak), mouse and rat (weak) leptin. Detects a band of ~16kDa by Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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