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You are here:Home » Antibodies » Abs to Leptin » Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Catalog #L1669-06A
Application(s)
ELISA (direct and indirect; 1:5000-1:10000)
Western Blot (1:5000-1:10000 using ECL; suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
Concentration1mg/ml
FormLiquid. Purified antibody in PBS, pH 7.4. Contains no preservatives.
PurityPurified
ImmunogenRecombinant mouse leptin.
SpecificityRecognizes human (weak), mouse and rat (weak) leptin. Detects a band of ~16kDa by Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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