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You are here:Home » Antibodies » Abs to Leptin » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B
Catalog #L1669-07
ApplicationsSuitable for use in ELISA, Western Blotting, Other applications not tested.
Recommended DilutionELISA (direct and indirect): 1:5000-1:10,000).
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at RT).
Optimal conditions must be determined individually for each application.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D524
HostMouse
SourceHuman
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
ImmunogenRecombinant human leptin:Fc expressed from mammalian cells.
SpecificityRecognizes both natural and recombinant human leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with mouse or rat leptin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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