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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin, aa131-145 (Ob, Obese Protein, Obesity Factor)

Anti -Leptin, aa131-145 (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IC
Applications: This product has been found to stain speci cally human adipocytes at a 1:1000 dilution. The ability to bind a leptin in adipocytes was examined in cells xed with neutral buffered formalin. The xed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated Goat anti-Mouse IgG. The immuno uorescent staining pattern was observed using epi uorescence microscopy. Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~16kD at a dilution of 1:4000
Catalog #L1670-01B
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.B.782
HostMouse
SourceHuman
ConcentrationNot determined
FormAscites
PurityNeat ascites
ImmunogenHuman leptin (131145) attached to a carrier protein
SpecificityRecognizes Human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin speci cally. Crossreactivity: Leptin (Human) 100% Leptin (131-145) 100% Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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