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Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)
Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E IH IF |
|
| Catalog # | L1670-01C | | Applications | Suitable for use in Immunofluorescence, ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:2000 The ability to bind a leptin in adipocytes was examined in cells xed with neutral buffered formalin. The xed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted antibody and then with FITC-conjugated Goat anti-mouse IgG. The immuno uorescent staining pattern was observed using epi uorescence microscopy. | | Immunohistochemistry: Frozen sections | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 1.B.784 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid. Preservative free. | | Purity | Neat ascites | | Immunogen | Synthetic human leptin (92–145) cyclized. | | Specificity | Recognizes human Leptin, cyclized (aa92-145). Binds to the carboxyl terminal region of the protein and to the intact leptin specifically. Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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