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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)

Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)


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Clone Host Grade Applications
Monoclonal Mouse Ascites E IH IF
Catalog #L1670-01C
ApplicationsSuitable for use in Immunofluorescence, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:2000 The ability to bind a leptin in adipocytes was examined in cells xed with neutral buffered formalin. The xed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted antibody and then with FITC-conjugated Goat anti-mouse IgG. The immuno uorescent staining pattern was observed using epi uorescence microscopy.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No1.B.784
ConcentrationNot determined
FormSupplied as a liquid. Preservative free.
PurityNeat ascites
ImmunogenSynthetic human leptin (92145) cyclized.
SpecificityRecognizes human Leptin, cyclized (aa92-145). Binds to the carboxyl terminal region of the protein and to the intact leptin specifically. Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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