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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)

Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IH IC IF
Catalog #L1670-02A
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionImmunocytochemistry: 0.25ug/ml. The ability to bind a leptin in adipocytes was examined in cells xed with neutral buffered formalin. The xed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated goat anti-mouse IgG.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
SourceAscites
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS 10mg/ml BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.B.785
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with 40-50% glycerol, PBS, 10mg/ml BSA.
PurityPuri ed IgG Fraction
ImmunogenHuman leptin (92145) cyclized.
SpecificityRecognizes human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin speci cally. Crossreactivity: Leptin (100%), Leptin (131-145) (100%). Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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