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Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)
Anti -Leptin, cyclized, aa92-145 (Ob, Obese Protein, Obesity Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E IH IC IF |
|
| Catalog # | L1670-02A | | Applications | Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. | | Recommended Dilution | Immunocytochemistry: 0.25ug/ml. The ability to bind a leptin in adipocytes was examined in cells xed with neutral buffered formalin. The xed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated goat anti-mouse IgG. | | Immunohistochemistry: Frozen | | Optimal dilutions to be determined by the researcher. | | Source | Ascites | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS 10mg/ml BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 1.B.785 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. Reconstitute with 40-50% glycerol, PBS, 10mg/ml BSA. | | Purity | Puri ed IgG Fraction | | Immunogen | Human leptin (92–145) cyclized. | | Specificity | Recognizes human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin speci cally. Crossreactivity: Leptin (100%), Leptin (131-145) (100%). Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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