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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #L1670-07D
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 using neat serum) with Chemiluminescence technique. The obese protein is predicted to be secretory protein with a molecular weight of ~ 16kD (refs. 1, 2).
ELISA: 1:10,000-50,000; 10-100ng of Leptin protein/well).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H31
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenPurified, human recombinant leptin
SpecificityMouse anti-human leptin reacts specifically with human. Weak crossreactivity may be seen with mouse/rat leptin. ADI has also made polyclonal anti-mouse and anti-human leptin. Antibody crossreactivity in various other species has not been studied
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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