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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #L1670-07K
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: > 0.5ug/ml using 100ul/well antibody solution allows the detection of 0.2-0.4ng/well of recombinant Leptin.
Western Blot: 0.1-0.2ug/ml; Detects recombinant Leptin at 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (prior to lyophilization)
FormSupplied as a lyophilized powder in 0.5X PBS, pH 7.4. Reconstitute with sterile water or 40-50% glycerol to a concentration of 0.2mg/ml
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure recombinant murine Leptin
SpecificityRecognizes murine Leptin. Species Sequence Homology: Rat - 96% (161/167).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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