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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #L1670-07K
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: > 0.5ug/ml using 100ul/well antibody solution allows the detection of 0.2-0.4ng/well of recombinant Leptin.
Western Blot: 0.1-0.2ug/ml; Detects recombinant Leptin at 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~1mg/ml (prior to lyophilization)
FormSupplied as a lyophilized powder in 0.5X PBS, pH 7.4. Reconstitute with sterile water or 40-50% glycerol to a concentration of 0.2mg/ml
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure recombinant murine Leptin
SpecificityRecognizes murine Leptin. Species Sequence Homology: Rat - 96% (161/167).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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