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Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Catalog # | L1670-07L | | Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilution | ELISA: 01.5-3ug/ml using 100ul/well antibody solution allows the detection of >0.2ng/well of recombinant Leptin. | | Western Blot: 0.1-0.2ug/ml Detects recombinant Leptin at 1.5-3ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, 0.1% BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | 1mg/ml | | Form | Supplied as a lyophilized powder in PBS, pH 7.4. Conjugated to Biotin. Reconstitute with sterile PBS with 0.1% BSA or 40-50% glycerol to a concentration of 0.05mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly pure recombinant murine Leptin | | Specificity | Recognizes murine Leptin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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