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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin, NT, aa1-24 (Ob, Obese Protein, Obesity Factor)

Anti -Leptin, NT, aa1-24 (Ob, Obese Protein, Obesity Factor)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Applications: Suitable for immunohistochemical and Western immunoblotting. This antiserum has been characterized by Western blot, ELISA and cell staining. This IgG fraction has been found to stain specifically human adipocytes at a 1:500 dilution. The ability of this antibody to bind to leptin in adipocytes was examined in cells fixed in neutral buffered formalin. The fixed cells were incubated for 20 minutes with 4% normal goat serum, reacted for 60 minutes with the diluted rabbit antibody, and then with FITC-conjugated goat anti-rabbit IgG. The immunofluorexscent staining pattern was observed using epifluorescence microscopy. Western immunoblots result in a single band at ~60kDa at a dilution of 1:1,000.
Catalog #L1670-08
Clone TypePolyclonal
ConcentrationNot determined
FormNeat, Lyophilized. Reconstitute with 0.1ml PBS containing 10mg/ml BSA for the equivalent of whole antisera or with additional buffer for more dilute antisera.
PuritySupplied as a sterile-filtered, defibrinated neat serum.
ImmunogenPeptide analogue of the amino terminal of human Leptin (1-24) covalently attached to a carrier protein.
SpecificityRecognizes Human Leptin. Binds to the amino terminal region of the protein and to the intact leptin speci cally. Crossreactivity: Leptin (Human) 100% Leptin (131-145) 100% Does not react with Human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Parathyroid hormone, Transforming Growth Factor-alpha and Rat Insulin-like Growth Factor 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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