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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Anti -Leptin (Ob, Obese Protein, Obesity Factor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #L1670-08B
Applications Suitable for use in ELISA and Western Blot. Other applications have not been tested.
 
Recommended Dilution:
ELISA (Indirect and Sandwich): 0.5-2ug/ml using 100ul/well antibody solution. In conjunction with compatible secondary reagents the detection is 0.20.4ng/well.
Western Blot: 0.10.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3ng/lane under either reducing or non-reducing conditions.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationNot determined.
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to 0.1-1mg/ml.
PurityPuri ed by af nity chromatography employing immobilized rat Leptin matrix.
ImmunogenHighly pure (>98%) recombinant rat Leptin.
SpecificityRecognizes rat Leptin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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