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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Leptin, a 16kD protein, is the product of the ob (obese) gene, is a 16kD protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue and is considered to play an important role in apetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.
Catalog #L1670-08C
Applications  
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA:  0.15-0.30ug/ml Detects rat Leptin by direct ELISA using 100ul/well Allows the detection of at least 0.2ng/well of recombinant rat Leptin.
Western Blot: 0.1-0.2ug/ml Allows the detection of recombinant rat Leptin at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS. Labeled with Biotin. Reconstitute with 40-50% glycereol, PBS, 0.1% BSA to a concentration of 0.1-1mg/ml
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (>98%) recombinant rat Leptin.
SpecificityRecognizes rat Leptin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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