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Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Anti -Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Leptin, a 16kD protein, is the product of the ob (obese) gene, is a 16kD protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue and is considered to play an important role in apetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function. | | | Catalog # | L1670-08C | | Applications | | | Suitable for use in ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilution | ELISA: 0.15-0.30ug/ml Detects rat Leptin by direct ELISA using 100ul/well Allows the detection of at least 0.2ng/well of recombinant rat Leptin. | | Western Blot: 0.1-0.2ug/ml Allows the detection of recombinant rat Leptin at 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS. Labeled with Biotin. Reconstitute with 40-50% glycereol, PBS, 0.1% BSA to a concentration of 0.1-1mg/ml | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly pure (>98%) recombinant rat Leptin. | | Specificity | Recognizes rat Leptin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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