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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin, aa92-145 (Ob, Obese Protein, Obesity Factor)

Anti -Leptin, aa92-145 (Ob, Obese Protein, Obesity Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E IC IF
Catalog #L1670-08G
ApplicationsImmunofluorescent Staining of Cells This monoclonal antibody has been found to stain specifically human adipocytes at a 1:25 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum, reacted for 60 minutes with the diluted mouse monoclonal antibody, and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescence microscopy.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No7C208
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenHuman leptin (92-145) cyclized
SpecificityBinds to the carboxyl terminal region of the protein and has been found to bind to intact leptin specifically in ELISAs and by immunocytochemistry. % Crossreactivity Leptin (Human)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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