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Anti -Leptin, aa92-145 (Ob, Obese Protein, Obesity Factor)
Anti -Leptin, aa92-145 (Ob, Obese Protein, Obesity Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
E IC IF |
|
| Catalog # | L1670-08G | | Applications | Immunofluorescent Staining of Cells This monoclonal antibody has been found to stain specifically human adipocytes at a 1:25 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum, reacted for 60 minutes with the diluted mouse monoclonal antibody, and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescence microscopy. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 7C208 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid. | | Purity | Supernatant | | Immunogen | Human leptin (92-145) cyclized | | Specificity | Binds to the carboxyl terminal region of the protein and has been found to bind to intact leptin specifically in ELISAs and by immunocytochemistry. % Crossreactivity Leptin (Human) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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