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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)

Anti -Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob,
Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Catalog #L1670-12J
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:4000. Detects a ~16kD protein representing leptin from 3T3-L1 cell extract.
Immunohistochemistry (Frozen): 1:1000.
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1 mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide correspoding to amino acids 25-44 [Q(25)KVQDDTKTLIKTIVTRIND(44)] of mouse leptin. This sequence is completely conserved between mouse and bovine. Cellular Localization: Secreted
SpecificityRecognizes mouse Leptin. Species Crossreactivity: human, rat, and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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