Recombinant protein corresponding to rat Leptin containing 147 amino acid residues, expressed in E. coli. Uniprot/Swiss Accession: P50596

MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC

Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile sterile dH2O with 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant, E. coli

Supplied as a lyophilized powder. Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.