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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor which regulates adipose-tissue mass/body weight through hypothalamic effects. The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.
Catalog #	L1671-03E
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:16,000
	Western Blot: 1.0-3.0ug/ml, detects a band of ~150kD in human brain cell lysates.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in TBS, pH7.3, 0.5% BSA, 0.02% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1;NP_001003680.1).
Specificity	Specific for human leptin receptor, designated CD295. Based on sequence similarity, is expected to react with canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.