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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin Receptor Gene Related Protein (OBGRP)

Anti -Leptin Receptor Gene Related Protein (OBGRP)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894 AA). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165 AA). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.
Catalog #L1671-24C
More recently, another variant of OBR, termed OB-RGRP (obese receptor gene related protein), has been identified that is distinct from the leptin receptor itself. OB-RGRP transcript contains the first two OBR gene 5'-untranslated exons, but then is alternatively spliced to two either leptin receptor or 131 aa protein OB-RGRP. The OB-RGRP is highly conserved in mouse, rat, human, yeast and C. elegans. OPB-RGRP is highly expressed in the hypothalamic regions that express the OBR mRNA. The OBR/B219, but not the OB-RGRP, is up-regulated in hypothalamus of ob/ob mice. OB-RGRP mRNA is also detected in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. A new full-length cDNA encoding a new 131aa protein, leptin receptor overlapping transcript-like 1 gene (LEPROTL1), that is highly homologous with OB-RGRP (~70% identity with OB-RGRP). LEPROTL1 is also widely expressed and has a similar JAK binding site (Pro46-Ile-Pro48) that is conserved in homologous proteins. LEPROTL1 protein is predicted to display 3 TM domains with an extracellualr N-terminus and intracellular C-terminus.
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern: 1:1000 dilution of antiserum or 1-10ug/ml of affinity purified IgG. Optimal dilutions must be optimized.
ELISA: 1:10,000-50,000 10-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
ImmunogenA 15 amino acid sequence within the first putative intracellular domain of human of human OB-RGRP (1) was coupled to KLH
SpecificityHuman sequence is 100% conserved in mouse, rat and human OB-RGRP. It is only 53% conserved in human LEPROTL1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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