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You are here:Home » Molecular Biology » Growth Factors-LIF » Leukemia Inhibitory Factor, Recombinant, Rat (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)

Leukemia Inhibitory Factor, Recombinant, Rat (LIF, Cholinergic Differentiation Factor,
CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL
Inhibitor, MLPLI)

Pricing

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Specifications

L2024-01R is an Escherichia coli­derived recombinant protein corresponding to full­length mature rat leukemia inhibitory factor (LIF), a secreted peptide with the capability to induce terminal differentiation in leukemic cells. It is expressed as a GST­fusion protein using a pGEX expression system, cleaved from GST via thrombin and purified by ion exchange chromatography.
Catalog #L2024-01R
Activity > 1x10e8 units/mg. 50 units is the amount of rat LIF required to induce differentiation in 50% of M1 colonies in 1ml agar cultures.
ApplicationsSuitable for use in Functional Assays. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAiquot and store undiluted at -20°C. Aliquots are stable for at least 6 months at -20°C. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight19.9kD
SourceE. coli
Purity> 85% by HPLC and SDS­PAGE analyses. Purified by ion exchange chromatography. Endotoxin: < 0.1ng/ug
Concentration~0.01mg/ml
FormSupplied as a liquid in PBS containing 1mM DTT, 10% glycerol, and 0.02% Tween 20
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEmfilermin


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