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Anti -Lewis A (Blood Group Lewis a, Lewis A Antigen)
Anti -Lewis A (Blood Group Lewis a, Lewis A Antigen)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. Increased expression of Lewis B has been reported in gastric and pancreatic tumors as well as in colorectal cancer. | | | Catalog # | L2053-02A | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (paraffin): 5ug/ml. Use sodium citrate for heat-mediated antigen retrieval. | | Immunohistochemistry (Frozen): 1:10-1:2000 | | ELISA: 1:2000 | | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8L586 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in in PBS, pH 7.4, 15mM sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Human tissue/cell preparation. Mucin isolated by size-exclusion chromatography from ovarian cyst fluid (pure endocervical type according to the Fenoglio’s classification). | | Specificity | Recognizes human Lewis A. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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