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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Lewis B (Blood Group Lewis b, Lewis B Blood Group Antigen)

Anti -Lewis B (Blood Group Lewis b, Lewis B Blood Group Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. Increased expression of Lewis B has been reported in gastric and pancreatic tumors, as well as in colorectal cancer.
Catalog #L2054-05C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (paraffin): 5ug/ml. Heat mediated antigen retrieval using sodium citrate is recommended. Incubate 1hour at RT.
Immunohistochemistry (Frozen): 1:10-1:2000
Western Blot: 1:1000
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L589
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenLewis B isolated from human ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
SpecificityRecognizes human Lewis B, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells and in secretions of certain individuals.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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