| Catalog # | L2075 |
| Applications | Suitable for use in Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry: 1:200-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Immunohistochemistry Protocol | 1. Dissect tissues. Freeze on dry ice. |
| 2. Cut on a cryostat ~10um slices. |
| 3. Dry slides at RT for 30-90 minutes. |
| 4. Fix with cold Acetone/Methanol (50/50) for 2 min then either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution (0.05% trypsin, incubation for 10 min. at 37°C, followed by 3 washes with PBS). |
| 5. Let air dry. |
| 6. Treat with PBS for 5 minutes. |
| 7. Incubate in 50mM ammonium chloride for 30 minutes. |
| 8. Treat with PBS for 5 minutes. |
| 9. Incubate with Blocking Serum for 30-45 minutes. |
| 10. Incubate with appropriately diluted L2075 for 90 minutes. |
| 11. Wash 3 times for 5 minutes. |
| 12. Incubate with fluorescent conjugated secondary anti-rabbit IgG for 30 minutes. |
| 13. Wash 3 times for five minutes. Mount. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. No preservative added. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa239-254 af human LHX2/Lim2. |
| Specificity | Recognizes the human Lim homeobox containing transcription factor LHX2. No significant sequence homology with other proteins. |
| Species Crossreactivity | Rodent. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
