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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -LHX2/Lim (Lim Region Containing Transcription Factor)

Anti -LHX2/Lim (Lim Region Containing Transcription Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH
Catalog #L2075
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol1. Dissect tissues. Freeze on dry ice.
2. Cut on a cryostat ~10um slices.
3. Dry slides at RT for 30-90 minutes.
4. Fix with cold Acetone/Methanol (50/50) for 2 min then either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution (0.05% trypsin, incubation for 10 min. at 37°C, followed by 3 washes with PBS).
5. Let air dry.
6. Treat with PBS for 5 minutes.
7. Incubate in 50mM ammonium chloride for 30 minutes.
8. Treat with PBS for 5 minutes.
9. Incubate with Blocking Serum for 30-45 minutes.
10. Incubate with appropriately diluted L2075 for 90 minutes.
11. Wash 3 times for 5 minutes.
12. Incubate with fluorescent conjugated secondary anti-rabbit IgG for 30 minutes.
13. Wash 3 times for five minutes. Mount.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa239-254 af human LHX2/Lim2.
SpecificityRecognizes the human Lim homeobox containing transcription factor LHX2. No significant sequence homology with other proteins.
Species CrossreactivityRodent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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