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You are here:Home » Culture Media » Media-Cell Culture, Leibowitz’s » Leibovitz L-15 Medium Modified, w/L-Glutamine, w/o MgCl2 (Powder)

Leibovitz L-15 Medium Modified, w/L-Glutamine, w/o MgCl2 (Powder)


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The most commonly used buffering system for mammalian cell cultures is a bicarbonate/CO2 system, which requires CO2 regulators and incubators to supply a constant level of CO2. As a replacement, Leibovitz developed a bicarbonate-free medium, L15, with relatively high levels of certain amino acids in the free base form.
Catalog #L2102
The most common method in use today for buffering mammalian cell cultures is the bicarbonate/CO2 system, which is based on the following equilibriumCO2 + H2O <-> HCO3" + H+
The use of bicarbonate is an attempt to mimic the buffering system of blood, but it has at least two major drawbacks. (1) The p/Q of bicarbonate is 6*1, which is far removed from the desired pH range of cell culture media (7-0-7-4). (2) Although bicarbonate is cheap, supplying a constant level of CO2 to cell cultures is definitely not, as it requires expensive CO2 incubators or fermentors.
AppearanceLight tan, homogeneous, free flowing powder
Solubility (14.1g/L)Red, clear, complete
pH7.4 ± 0.4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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