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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141)

Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein
Lhx1, MGC126723, MGC138141)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators and proto-oncogenes products. LIM domain mediates protein-protein interactions and, with LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein. This transcription factor is required for embryonic survival and for the determination of many cell types.
Catalog #L2200-01M
ApplicationsWestern blot analysis of LIM-1 in muscle lysate. A protein band of an approximate molecular weight of 60kD was detected. Human muscle lysate can be used as a positive control and a band at 60kD will be detected.
Recommended DilutionWestern blot: 0.25ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides corresponding to amno acids 149-165, 175-162, and 395-412 of human Lim-1.
SpecificityRecognizes human LIM-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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