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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

LIM kinases (LIMK1 and LIMK2) are serine/ threonine kinases that have two zinc finger motifs, known as LIM motifs, in their N-terminal regulatory domain (1). LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 in the activation loop, which increases the activity of the kinases (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminal serine 3 residue of cofilin (6,7).
Catalog #	L2212
Applications	Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to the C-terminal residues of human LIMK1 (KLH coupled).
Specificity	Detects endogenous levels of total LIMK1 protein independent of phosphorylation. Does not crossreact with LIMK2. Species Crossreactivity: Human mouse, rat and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.