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You are here:Home » Antibodies » Antibodies-Transcription Factors, Zinc (Ring) Finger » Anti -LIM Kinase 1, Phosphorylated (Thr508)/LIM Kinase 2 (Thr505)

Anti -LIM Kinase 1, Phosphorylated (Thr508)/LIM Kinase 2 (Thr505)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
LIM kinases (LIMK1 and LIMK2) are serine/ threonine kinases that have two zinc finger motifs, known as LIM motifs, in their N-terminal regulatory domain (1). LIM kinases are involved in actin cytoskeletal regulation from Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at conserved threonines 508 or 505 in the activation loop, which increases the activity of the kinases (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminal serine 3 residue of cofilin (6,7).
Catalog #L2212-05
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:25
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA, glycerol. See L2212-05A.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Thr508 of human LIMK1 (KLH coupled).
SpecificityDetects overexpressed LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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