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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

LIM kinases (LIMK1 and LIMK2) are serine/ threonine kinases that have two zinc finger motifs, known as LIM motifs, in their N-terminal regulatory domain (1). LIM kinases are involved in actin cytoskeletal regulation through Rho-family GTPases and downstream kinases PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at conserved threonines 508 or 505 in the activation loop, which increases the activity of the kinases (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminal serine 3 residue of cofilin (6,7).
Catalog #	L2214
Applications	Suitable for use in Western Blotting. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Form	Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50% glycerol.
Purity	Purified by protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide (KLH coupled) corresponding to C-terminal residues of human LIMK2.
Specificity	Detects overexpressed levels of LIMK2. Does not crossreact with LIMK1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.