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Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)
Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3,
Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related
subunit of MMP-9)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Lipocalin 2 is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. Lipocalin 2 is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of Lipocalin 2 in a variety of epithelia may be increased in inflammation or cancers (2). Expression of Lipocalin 2 in the kidney is dramatically increased by acute kidney injury (3). | | | Catalog # | L2499-16 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | ELISA: Strong reaction seen when used as detection antibody in sandwich ELISA in combination with a polyclonal Lipocalin 2 capture antibody and when tested on recombinant Lipocalin 2 coated directly onto the well. | | Western Blot: 1:50. Reacts with Lipocalin 2 in both reduced and unreduced forms in Western blotting of SDS-PAGE gels. | | Immunohistochemistry: Reacts with Lipocalin 2 when staining paraformaldehyde-fixed sections containing neutrophils. | | Optimal dilutions to be determined by the researcher. | | Culture Medium | RPMI 1640 with 10% fetal calf serum. | | Hybridoma | X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6F155 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant protein corresponding to human Lipocalin 2 in monomer and dimer forms adsorbed to aluminium hydroxide gel. | | Specificity | Recognizes human Lipocalin 2. Epitope specificity differs from that of clone 6F154. Species Crossreactivity: Cynomolgus monkey (Macaca fascicularis) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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