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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)

Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3,
Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related
subunit of MMP-9)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
Lipocalin 2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1-3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9nM) (4). Secretion of Lipocalin 2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin 2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).
Catalog #L2499-18
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA (Capture): 0.2-0.8ug/ml
Western Blot: 1ug/ml detects Lipocalin 2 in rat ovary tissue and mouse lung tissue.
Immunoprecipitation: 25ug/ml immunoprecipitates Lipocalin 2 from conditioned cell medium spiked with recombinant rat Lipocalin 2.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa21-198 from rat Lipocalin 2 expressed in NSO cells (P30152).
SpecificityRecognizes rat Lipocalin 2 at ~25kD. Shows <0.4% crossreactivity in sandwich ELISAs with recombinant mouse Lipocalin 2 and <0.2% crossreactivity with recombinant human Lipocalin 1 and Lipocalin 2. Species Crossreactivity (Western Blot): mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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