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Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)
Anti -Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3,
Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related
subunit of MMP-9)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IP |
|
| Lipocalin 2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1-3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9nM) (4). Secretion of Lipocalin 2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin 2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8). | | | Catalog # | L2499-18 | | Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | ELISA (Capture): 0.2-0.8ug/ml | | Western Blot: 1ug/ml detects Lipocalin 2 in rat ovary tissue and mouse lung tissue. | | Immunoprecipitation: 25ug/ml immunoprecipitates Lipocalin 2 from conditioned cell medium spiked with recombinant rat Lipocalin 2. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Rat | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant protein corresponding to aa21-198 from rat Lipocalin 2 expressed in NSO cells (P30152). | | Specificity | Recognizes rat Lipocalin 2 at ~25kD. Shows <0.4% crossreactivity in sandwich ELISAs with recombinant mouse Lipocalin 2 and <0.2% crossreactivity with recombinant human Lipocalin 1 and Lipocalin 2. Species Crossreactivity (Western Blot): mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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