| Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)- |
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| cholesterol plays a central role in various disease developments. It is well known that low |
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| levels of HDL and high level of LDL are associated with an increased risk of cardiovascular |
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| events. The HDL and LDL/VLDL Cholesterol Quantification Kit provides a simple |
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| quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL |
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| and VLDL (very low-density lipoprotein) in serum samples. In the assay, cholesterol oxidase |
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| specifically recognizes free cholesterol and produces products which react with probe to |
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| generate color (lambda = 570nm) and fluorescence (Ex/Em = 538/587nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free |
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| cholesterol can be detected separately in the presence and absence of cholesterol esterase |
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| in the reactions. |
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| Catalog # | L2600-56 |
| Kit Components | Cholesterol Assay Buffer 1x25ml |
| 2X LDL/VLDL Precipitation Buffer 1x10ml |
| Cholesterol Probe (in DMSO, anhydrious) 1x200ul |
| Enzyme Mix (Lyophilized) 1x1vial |
| Cholesterol Esterase (Lyophilized) 1x1vial |
| Cholesterol Standard (2ug/ul) 1x100ul. |
| Storage and Stability | Store the kit at -20°C, protect from the light. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
