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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Lipoprotein Receptor-related Protein 6, Low Density (LRP6, FLJ90062, FLJ90421)

Anti -Lipoprotein Receptor-related Protein 6, Low Density (LRP6, FLJ90062, FLJ90421)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP
LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density-lipoprotein re-eptor (LDLR) related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/beta-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490 and Thr1493 by kinases such as GSK-3 and CK1 (8-10). The phosphorylated LRP6 recruits axin to the membrane, and presumably activates beta-catenin signaling (8-10).
Catalog #L2601-06F
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10E36
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to an extracellular region surrounding
Met988 of human LRP6 protein (KLH).
SpecificityRecongnizes endogenous levels of human total LRP6 protein. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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