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You are here:Home » Antibodies » Abs to Leukotrienes » Anti -LOX-1 (CLEC8A, hLOX-1, Lectin-like Oxidized LDL Receptor 1, Lectin-like OxLDL Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOX1, LOX-1, Oxidized Low Density Lipoprotein (Lectin-like) Receptor 1, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL Rece

Anti -LOX-1 (CLEC8A, hLOX-1, Lectin-like Oxidized LDL Receptor 1, Lectin-like OxLDL
Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOX1, LOX-1, Oxidized Low Density
Lipoprotein (Lectin-like) Receptor 1, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL
Rece

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC IF
LOX-1 is a single-pass type II membrane protein (~45kD) and belongs to the C-type lectin-like protein superfamily. LOX-1 is expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver, brain aortic intima, bone marrow, spinal cord and substantia nigra. It is also expressed on the surface of dendritic cells.
This unique scavenger receptor LOX-1 plays important roles in atherogenesis. LOX-1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (OxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis, inducing vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. LOX-1 associates with OxLDL inducing the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding OxLDL, LOX-1 acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. LOX-1 is involved in the inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. LOX-1 also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
The LOX-1 gene is a so-called immediate early gene that is dynamically modulated by several factors in vitro and in vivo. LOX-1 expression is induced by stimuli such as inflammatory cytokines, OxLDL, TNF-alpha, TGF-beta, and ANG II in vitro, and several proatherogenic factors in vivo.
Catalog #L2623-01B
ApplicationsSuitable for use in Flow Cytometry and Functional Studies. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50; Used on peripheral blood myeloid DC and macrophages.
Functional Studies: Antibody completely prevented HSP70 binding to LOX-1-CHO but not mock-transfected CHO cells (1).
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlHuman APCs
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G220
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenA recombinant corresponding to the extracellular domain (aa71-273) of human LOX-1 with murine Fcg1 (LOX-1-muFc) produced in PEAK cells.
SpecificityRecognizes human lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Neutralizes LOX-1 and inhibits Hsp70 binding to dendritic cells and Hsp70-induced antigen cross-presentation. In vivo, targeting LOX-1 with a tumor antigen using L2623-01B induces anti-tumor immunity. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSCARE1, CLEC8A, OLR3


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