Anti -LOX-1 (CLEC8A, hLOX-1, Lectin-like Oxidized LDL Receptor 1, Lectin-like OxLDL
Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOX1, LOX-1, Oxidized Low Density
Lipoprotein (Lectin-like) Receptor 1, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL
Rece
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC IF |
|
| LOX-1 is a single-pass type II membrane protein (~45kD) and belongs to the C-type lectin-like protein superfamily. LOX-1 is expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver, brain aortic intima, bone marrow, spinal cord and substantia nigra. It is also expressed on the surface of dendritic cells. | | | This unique scavenger receptor LOX-1 plays important roles in atherogenesis. LOX-1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (OxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis, inducing vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. LOX-1 associates with OxLDL inducing the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding OxLDL, LOX-1 acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. LOX-1 is involved in the inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. LOX-1 also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. | | | The LOX-1 gene is a so-called immediate early gene that is dynamically modulated by several factors in vitro and in vivo. LOX-1 expression is induced by stimuli such as inflammatory cytokines, OxLDL, TNF-alpha, TGF-beta, and ANG II in vitro, and several proatherogenic factors in vivo. | | | Catalog # | L2623-01B | | Applications | Suitable for use in Flow Cytometry and Functional Studies. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1:50; Used on peripheral blood myeloid DC and macrophages. | | Functional Studies: Antibody completely prevented HSP70 binding to LOX-1-CHO but not mock-transfected CHO cells (1). | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Control | Human APCs | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9G220 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | A recombinant corresponding to the extracellular domain (aa71-273) of human LOX-1 with murine Fcg1 (LOX-1-muFc) produced in PEAK cells. | | Specificity | Recognizes human lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Neutralizes LOX-1 and inhibits Hsp70 binding to dendritic cells and Hsp70-induced antigen cross-presentation. In vivo, targeting LOX-1 with a tumor antigen using L2623-01B induces anti-tumor immunity. Species Crossreactivity: mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SCARE1, CLEC8A, OLR3 |
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