Anti -Lithostathine-1-alpha (REG1A, Islet Cells Regeneration Factor, ICRF, Islet of
Langerhans Regenerating Protein, REG, Pancreatic Stone Protein, PSP, Pancreatic Thread
Protein, PTP, Regenerating Islet-derived Protein 1-alpha, REG-1-alpha, Regenerating Protei
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B IH |
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| Reg1A is a secreted, variably glycosylated 15-22kD type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet B-cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/ml quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166aa precursor. It contains a 22aa signal sequence plus a 144aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain aa34-164 and a utilized N-terminal O-linked glycosylation site 27aa. Variablility in the glycosylation pattern, plus proteolytic cleavage after 11aa generates Reg1A isoforms in the 15-22kD range. There is one potential isoform variant that shows an alternative start site at Met9. Mature aa23-166 human Reg1A shares 76% and 69%aa sequence identity with mouse and rat Reg1A, respectively. | | | Catalog # | L3010-19A | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Immunohistochemistry: 5-15ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.1mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa23-166 from human Reg1A.expressed in CHO cell line. | | Specificity | Recognizes human Reg1A. Species sequence homology: recombinant human Reg1B (80%), recombinant mouse Reg1 (20%), rhReg3A and recombinant rat Reg1A (5%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Lithostathine-1-alpha (REG1A, Islet Cells Regeneration Factor, ICRF, Islet of Langerhans Regenerating Protein, REG, Pancreatic Stone Protein, PSP, Pancreatic Thread Protein, PTP, Regenerating Islet-derived Protein 1-alpha, REG-1-alpha, Regenerating Protei |
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